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Image Search Results
Journal: PLoS Biology
Article Title: A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation
doi: 10.1371/journal.pbio.3001361
Figure Lengend Snippet: (A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a DeltaVision deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Article Snippet: Samples were imaged with either a
Techniques: Standard Deviation, Flow Cytometry, Stable Transfection, Microscopy, Western Blot