deltavision elite imaging system Search Results


96
GE Healthcare deltavision system
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GE Healthcare deltavision ultra microscopic imaging system
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Ultra Microscopic Imaging System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
GE Healthcare deltavision elite high resolution microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Elite High Resolution Microscope, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Applied Precision Inc deltavision imaging system
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Imaging System, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TOPTICA Photonics deltavision omx sr imaging system
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Omx Sr Imaging System, supplied by TOPTICA Photonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Nikon a1r confocal microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
A1r Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Softworx Inc deltavision software softworx
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Software Softworx, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss axiovert-deltavision image restoration microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Axiovert Deltavision Image Restoration Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss deltavision restoration microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Restoration Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss lsm800 microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Lsm800 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Applied Precision Inc deltavision core microscope
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Deltavision Core Microscope, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss microscopes zeiss axiovert
(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a <t>DeltaVision</t> deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.
Microscopes Zeiss Axiovert, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microscopes zeiss axiovert - by Bioz Stars, 2026-07
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Image Search Results


(A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a DeltaVision deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.

Journal: PLoS Biology

Article Title: A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation

doi: 10.1371/journal.pbio.3001361

Figure Lengend Snippet: (A) Left: GFP-RNF152 protein levels after MG132 (50 μM), BafA1 (400 nM), and double treatment for 6 hours. Right: long exposure. (B) Quantification of the full-length GFP-RNF152 protein level in A, n = 3. Error bars represent standard deviation. n.s., not significant. ** p ≤ 0.01, *** p ≤ 0.001. (C) Quantification of the total GFP-RNF152 protein level, including full-length and the high molecular mass smear in A, n = 3. (D) Accumulation of polyubiquitinated GFP-RNF152 after the BafA1 and BafA1+MG132 treatments. (E) Flow cytometry analysis of stably express GFP-RNF152 in HEK293 cells after 6 hours of indicated treatments. (F) Localization of GFP-RNF152 and LAMP2 after indicated treatments. Images were taken by a confocal microscope. (G) Localization of GFP-RNF152 and LAMP2 after BafA1 (400 nM) treatment. The image was taken by a DeltaVision deconvolution microscope. Line scan analysis highlighted the lumenal localization of GFP-RNF152. Uncropped western blots and microscopy images are provided in . The numerical data underlying the plot can be found in . WT, wild-type.

Article Snippet: Samples were imaged with either a DeltaVision system (GE Healthcare Life Sciences) or a Leica SP8 confocal microscope system [ ].

Techniques: Standard Deviation, Flow Cytometry, Stable Transfection, Microscopy, Western Blot